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Co localization Experiment

Four-Channel Merged Image

The Fluorescence CV extension to IPLab/Mac provides a quick and easy way to measure the co localization of different fluorescent probes. The extension can compare single images and/or sequences of images. In addition, Fluorescence CV provides more advanced tools for the reproducible measurement and analysis of co localization experiments than provided for by simple ROI methods.

Complete with a split view window, Fluorescence CV allows a dynamic view of co localized areas when different experiment parameters are adjusted. Fluorescence CV comes complete with full statistical analysis, data export, and scatter plots with relevant experimental parameters displayed.

Color Join

The Color Join feature is used with multi-mode imaging experiments. Fluorescence CV can merge as many as nine fluorescence images and one bright-field image (DIC or phase) to make a single color image. This is in addition to the core program's three-channel capability.

Each fluorescent channel may be assigned a custom color to reflect the specific fluorescent probe used in the experiment. Color Join also supports the joining of multiple time-lapse image sequences or Z-stacks. Individual image channels may also be corrected by pixel shifting if required due to light path registration problems.