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With Ratio Plus, you can acquire image sequences, store them in memory or on hard disk, calculate ion concentration, plot mean fluorescence or ion concentration over time, and animate the resulting image sequence. In addition, Ratio Plus gives you maximum flexibility in designing your own experiments.
Ratio Plus systems can use digital or intensified-video CCD cameras. The ICCD-based system is designed specifically for ratio imaging of fast cellular events. Because this system utilizes an intensified CCD video camera, you can detect very low light emissions from your fluorescent samples even at video-rate exposure times (1/30 of a second). The software can perform image averaging or summing to improve the overall signal to noise ratio (SNR) and the dynamic range of your intensity readings.
You can also choose a cooled digital CCD system which is very versatile, although somewhat slower. Digital CCD cameras give higher spatial resolution in the XY plane. The cooling provides greater signal-to-noise performance, while the 12-bit digitization yields greater gray level resolution to detect minute changes in ion concentration.
Add a high-speed filter wheel or other wavelength changer, and you can rapidly capture image data resulting in high quality time-lapse sequences of dual-wavelength images. Switch between using the excitation or the emission filter wheel, or use both. Ratio Plus’ acquisition capability includes control of LEP, Prior, or Sutter filter wheels and shutters, or most other RS-232-controllable wavelength changing devices. This gives you the control of exposure at the excitation light source, minimizing photobleaching and subsequent damage to the sample.
