Microscope Illumination Archives - Digital Imaging Systems

Prior Lumen 1600 Multi-channel LED system

Becky — Tue, 31 Jan 2017 12:03:15 +0000

The Lumen 1600 provides high quality LED light for a vast range of applications. Provided with 16 LEDs, together covering the spectrum from 365 nm to 770 nm, the Lumen 1600 can provide either wide spectrum white light, or up to four narrow wavelengths of light, depending on the needs of the user. Ideal for advanced research and core facilities, the Lumen 1600 is flexible, easy to use, and designed to produce the very best in fluorescent imaging. Using an innovative four channel system, in which LEDs of similar wavelengths are placed in the same channel, up to four distinct flurophores can be excited simultaneously. The groupings allow the use of almost all stains used in multi-band combinations allowing great flexibility in experimental work.

Key features:

Flexible and easy to use
Excellently excites most common fluorophores
Can excite up to four distinct flurophores simultaneously – ideal for complex multi-band work
Compatible with all commercially available dual, triple and quadruple filters
LEDs offer fine control (in 1% increments), instant switch-on, consistent light, and minimal heat and vibration production, with an operational lifetime of at least 25,000 hours
LED intensity can be altered individually.
Multiple pre-set configurations can be created, stored and used.

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Prior Lumen 100 LED Illuminator

Becky — Tue, 31 Jan 2017 12:00:13 +0000

The Lumen 100 illumination system consists of a control box, an LED and a microscope specific adapter. A combiner is available if another LED unit is to be added. The LED directly couples to the microscope for maximum light transmission efficiency. 10 different LEDs emitting at specific wavelengths are available, from 365 nm to 660 nm, as well as a white light emitting LED. Excitation filters can be added to each LED to optimise the bandwidth of light for the specific application.

Key features:

365, 385, 405, 447, 460, 505, 525, 590, 625, 660 and white light LEDs available.
The LED bulbs have a long operational lifespan, do not contain mercury and produce minimal heat and vibration
Precise control of illumination is available in 1% increments
Instant switch on and off capability

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Prior LDB100F Brightfield LED with 550nm Filter

Becky — Tue, 31 Jan 2017 11:55:26 +0000

Prior Scientific is proud to announce the latest addition to their wide range of illumination products for microscopy – the new LDB100/101F Brightfield LLDB100F with FLIP IN 550 nanometer filter in the light path, which eliminates any background fluorescence. The LDB100F Brightfield LED illuminator provides all the advantages of LED illumination in a flexible package that can be fitted to most modern upright and inverted microscope systems.

Key features:

10,000 hours operating lifetime
Filter in for fluorescence applications eliminating unwanted background fluorescence
Filter out for brightfield applications, full spectrum
Fits most modern upright and inverted microscope systems
Replaces standard lamp houses
Choose between manual intensity, TTL control or on/off which eliminates the need for shutter mechanisms
Compatible with ProScan and OptiScan controllers
Adapters are available for most modern microscopes
Can be utilized as a standalone light source
<140 microseconds on/off speed using TTL

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Prior Lumen 300-LED White Light Fluorescence Excitation Illumination System

Becky — Tue, 31 Jan 2017 11:52:23 +0000

The new Lumen 300-LED is Prior Scientific’s latest addition to their wide range of illumination products for microscopy. The new Lumen 300-LED from Prior Scientific is the latest product in Prior’s ever expanding microscopy illumination product line. The unit is specifically designed to offer broad spectrum LED illumination for fluorescence applications. Fitting directly to most microscopes, the system is simple to install and use. Controlled by a manual keypad controller, the Lumen 300-LED offers instant on/off operation via TTL, manual 0-100% intensity control in 1% increments and on/off control for each LED. An optional liquid light guide is available when there is a need to keep the illumination source remote from the microscope. Providing 25,000+ hours of bulb life, the Lumen 300-LED is an environmentally friendly illumination system that requires no special disposal regulations.

Key Features:

Broad spectrum LED illumination for fluorescence applications.
Fits directly to most microscopes.
Simple to install and simple to use.
Instant on/off via TTL
Optional liquid light guide when there is a need to keep the source of illumination remote from the microscope.
Environmentally friendly:
No Mercury
No special disposal regulations
The manual keypad controller offers:
0-100% intensity control in 1% increments.
Independent brightness and on/off control for each LED.

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Prior Lumen 200S Fluorescence Illumination Systems with Shutter Control

Becky — Tue, 31 Jan 2017 11:48:12 +0000

The Prior Scientific Lumen 200S is a powerful, cost effective alternative to standard 100 Watt mercury and halogen lamp houses. With a minimum exposure time of 20 milliseconds, the Lumen 200S is suitable for the most demanding imaging applications. A wide variety of control options are available for the Lumen 200S including RS232, USB, TTL and via the ProScan III shutter port. Designed to mount remotely via a two or three meter liquid light guide the Lumen 200S eliminates heat transfer to the microscope, minimizing thermal drift and aiding image stability in long term experiments.

Key Features:

2,000 hour life, 200 Watt metal arc lamp
Remote mounting with two or three meter light guide
Stabilized DC power supply for consistent illumination
Easy to view display screen for bulb life indication and alarm description
Knurled light guide clamping knob for secure light guide installation
Adapters available for all modern research microscopes
Six position adjustable aperture/intensity knob to protect samples from photobleaching
Easy bulb replacement

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Prior Lumen 200/220PRO Fluorescence Illumination Systems with Integral Filter

Becky — Tue, 31 Jan 2017 11:45:23 +0000

For fully automated solutions, the Lumen 200PRO features a motorized internal six position filter wheel for fast switching of excitation wavelength. Being remote from the microscope means no vibration. No vibration means no settling time. No settling time means faster imaging! The light attenuation and shuttering is also motorized in the Lumen 200PRO allowing a selection of intensity over 100 increments to perfectly balance exposure to the sensitivity of your cells and fluorescent markers.

Prior also offers the Lumen 220PRO model which offers extended transmission in UV and IR compared to the standard Lumen 200PRO model.

Key Features:

2,000 hour life, 200 Watt metal arc lamp
Stabilized DC power supply for consistent illumination
Quiet operation suitable for laboratory use
Easy to view display screen for bulb life indication and alarm description
Knurled light guide clamping knob for secure light guide installation
Adapters available for all modern research microscopes
Pre-aligned bulb for easy bulb replacement
Integral high speed motorized six position 25mm diameter filter wheel
High speed motorized light attenuator, 0-100% in 1% increments
Compatible with ProScan™ III controllers

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Prior Lumen 200/220 Fluorescence Illumination

Becky — Tue, 31 Jan 2017 11:41:29 +0000

The Lumen 200 Fluorescence Illumination System is a powerful, cost effective alternative to standard 100 Watt mercury and halogen lamp houses. The combination of Prior’s proprietary 2,000 hour life, 200 watt metal arc lamp and two or three meter liquid light guide provide unrivalled versatility and illumination power. The Lumen 200 utilizes a metal halide lamp rather than the more traditional high pressure mercury or xenon burners. This means 2000 hours of stable output compared to a maximum of 300 hours for a mercury lamp. Coupled to the microscope using a liquid light guide there is very little heat transfer helping to eliminate thermal drift during time lapse experiments. The Lumen 200 also incorporates a variable light attenuation control to reduce the potential of bleaching and phototoxicity.

Prior also offers the Lumen 220 model which offers extended transmission in UV and IR compared to the standard Lumen 200 model. Prior Scientific also offers the Lumen 220 Fluorescence Illumination System with extended wavelength in UV and IR. Fluorophores requiring light in the 360nm and below range will see significant increases in signal strength from the Lumen 220 as compared to the Lumen 200 models.

Key Features:

2,000 hour life, 200 Watt metal arc lamp
Remote mounting with two or three meter light guide
Stabilized DC power supply for consistent illumination
Quiet operation suitable for laboratory use
Easy to view display screen for bulb life indication and alarm description
Knurled light guide clamping knob for secure light guide installation
Adapters available for all modern research microscopes
Six position adjustable aperture/intensity knob to protect samples from photobleaching
Easy bulb replacement

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CoolLED pE-100wht LED Illumination

Becky — Mon, 30 Jan 2017 14:40:28 +0000

The pE-100wht is a powerful white LED illumination system designed to replace a 100W halogen lamp.

Histologists and cytologists who are familiar with results from a conventional halogen lamp can immediately make accurate and reliable diagnosis using the pE-100wht as colours will appear the same.

Unlike a Halogen lamp, the colour balance of the pE-100wht illumination system does not vary with intensity, removing the need to make any adjustment.

The many additional benefits which LEDs offer – mercury-free, instant on/off, control and repeatability – mean that laboratories using halogen lamps will find that the move to the pE-100wht is obvious and simple.

Key Features:

Spectral performance matched to 100W halogen – familiar images
No variation in colour across intensity range – no need to colour balance
Replaces 100W halogen lamp housing on most current and older microscopes – quick & easy exchange
No unwanted UV – reduced risk of eye damage
Instant on/off – no shutters required, no warm up or cool down
Simple to fit, simple to use – no need to align
Stable & repeatable – reliable and consistent results
Wide range of microscope adaptors – fits most microscopes
Precise intensity control in 1% steps (0-100%) – no ND filters required
Excellent uniformity over field of view – fixed and stable, no alignment necessary
Long lifetime – expected to exceed 50,000 hours of operating time

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CoolLED pE-2 LED Illumination

Becky — Mon, 30 Jan 2017 14:35:11 +0000

The pE-2 is a fluorescence LED illumination system which provides repeatable and controllable light with a choice of 20 different LED peaks. It is perfect for the researcher who has a specific set of tests to conduct.

The user can specify a wavelength configuration matched to their particular fluorescence requirements. It is modular, allowing LED wavelengths to be exchanged as requirements vary or additional experiments are added.

Manual control is via a remote control pod with instant on/off and intensity control from 0-100%. Additionally, the system is fully integrated under many imaging software packages.

Key Features:

Modular system which drives up to 4 wavelengths – suitable for all fluorescence experiments
Integrated with many imaging software packages – perfect for automation and time-lapse
Collimator accepts excitation filters for fast-switching applications – filter wheel not required
Instant on/off – no shutters required, no warm up or cool down
Simple to fit, simple to use – no alignment, a once only adjustment
Stable & repeatable – reliable and consistent results
Wide range of microscope adaptors – fits most microscopes
Precise intensity control in 1% steps (0-100%) – no ND filters required
Excellent uniformity over field of view – fixed and stable, no alignment necessary
Long lifetime – expected to exceed 25,000 hours of operating time

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CoolLED pE-4000 LED Illumination

Becky — Mon, 30 Jan 2017 14:31:34 +0000

The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy. The system has 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope, making it the broadest spectrum of illumination available.

For the user who wants consistency and ease of use with simple on/off and intensity buttons, the pE-4000 can be operated as a ‘White’ illumination system.

For the user who wants total control over the excitation source to improve imaging, individual wavelengths settings can be selected and controlled in “Advanced” mode.

Light is delivered to the microscope by either a single liquid light guide (LLG) or fibre. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.

Key Features:

Broadest LED spectrum available: 365-770nm – match LED peaks to existing microscope filters
Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters
Individual control of selected LED wavelengths – less background, no filter wheel or shutters
Integrated with many imaging software packages – can be incorporated into all experimental parameters
Instant on/off – no shutters required, no warm up or cool down
Stable & repeatable – reliable and consistent results
Wide range of Microscope Adaptors – fits most microscopes
Precise intensity control in 1% steps (0-100%) – no ND filters required
Excellent uniformity over field of view – fixed and stable, no alignment necessary
Long lifetime – expected to exceed 25,000 hours of operating time
Pre-sets allowing lab manager to match white spectrum to existing filter cubes
Higher contrast images from matched-white spectrum
Individual LED wavelength selection
Rapid switching between LED wavelengths enables capture of high speed events
TTL & USB interfaces with imaging packages
Excitation filters can be fitted in optical path for controlled switching with no moving parts
Analogue inputs for dynamic intensity control
Optical feedback for applications requiring higher stability
Internal function generator for electrophysiology and optogenetics applications

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