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Photometrics Prime 95B sCMOS

Photometrics Prime 95B sCMOS 1.4 Megapixel Back Illuminated Cooled Scientific CMOS USB 3.0 Camera

1.4 Megapixel Back Illuminated Cooled Scientific CMOS USB 3.0 Camera

95% QE Backside Illuminated Scientific CMOS, when discovery depends on every photon

The study of live cell dynamics is often compromised by the poor throughput of optical systems. Spinning disk confocal microscopy is an example where limited optical throughput compromises frame rates as exposure times must be increased to compensate. Higher excitation intensity can be used but at a cost of accelerating phototoxicity and photobleaching.

The Prime 95B Scientific CMOS camera allows you to overcome many difficult imaging challenges by converting nearly every available photon to useful signal through an incredible 95% Quantum Efficiency – by using the first available scientific CMOS sensor with backside illumination. The result is the most sensitive CMOS camera available for scientific imaging. The Prime 95B gives you more signal and better experimental results with these exceptional features:

Key Features:

  • 95% Quantum Efficiency
  • 11µm x 11µm Pixel Area
  • 1200 x 1200 array – 1.44 Megapixel
  • 6e- Read Noise
  • 80,000e- Pixel Full Well
  • 61,500:1 Dynamic Range
  • 41fps @ 16-bit
  • 82fps @ 12-bit


Primary Applications:

  • Super-Resolution Microscopy
  • Single Molecule Fluorescence
  • Light Sheet Microscopy
  • Confocal Microscopy
Camera Specifications
Sensor TypeGPixel GSense 144 BSI CMOS gen IV, Grade 1 in imaging area
CCD Array1200 x 1200 pixels
Pixel Size11 x 11 µm
Sensor Dimensions13.2mm x 13.2mm (18.7mm Diagonal)
Peak Quantum Efficiency>95%
Read Noise1.6e- (Median), 1.8e- (RMS)
Full Well Capacity80,000e- (Combined Gain)
4,500 e- (High Gain)
Dynamic Range50,000:1 (Combined Gain)
Bit Depth16-bit (Combined Gain)
12-bit (High Gain)
Readout ModeRolling Shutter, Effective Globe Shutter
Supported Binning Modes2x2 (on FPGA)
Air Cooled-10°C @ 30°C Ambient
Dark Current 2.9e-/pixel/s
Liquid Cooled-25°C @ 30°C Ambient
Dark Current 0.7e-/pixel/s
Data InterfacePCI-Express
Optical InterfaceC-mount
Mounting Points2 x 1/4 20” mounting points per side to prevent rotation
Input Trigger ModesTrigger-First – Sequence triggered on first rising edge
Edge – Each frame triggered on rising edge
SMART Streaming – Fast iteration through multiple exposure times
Output Trigger ModesFirst Row – Expose signal is high while first row is acquiring data
Any Row – Expose signal is high while any row is acquiring data
All Row – Effective Globe Shutter -Expose signal is high when all rows are acquiring data
Output Trigger SignalsExpose Out (up to four signals), Read Out, Shutter Out, Trigger Ready

Frame Rates

Array Size16-bit12-bit
1200 x 12004182
1200 x 51296192
1200 x 256192384
1200 x 128384768